Representative Liz Cheney of Wyoming is highlighting her role as the top Republican on the Jan. 6 committee in a closing ad for her all but doomed re-election campaign, as polls show her badly trailing her Trump-backed opponent, Harriet Hageman, just five days before the primary.

But the nearly two-and-a-half-minute ad released online Thursday appeared aimed as much at a national audience as at the Republican primary voters in Wyoming who will decide the fate of Ms. Cheney, the state’s lone member of the House.

“The lie that the 2020 presidential election was stolen is insidious,” Ms. Cheney said as the ad opens. “It preys on those who love their country. It is a door Donald Trump opened to manipulate Americans to abandon their principles, to sacrifice their freedom, to justify violence, to ignore the rulings of our courts and the rule of law.”

Ms. Cheney, who has been vilified by former President Donald J. Trump and many of his supporters, defended the work of the special House committee that is investigating the 2021 attack on the Capitol and efforts by former Mr. Trump to overturn the 2020 election results.

Ms. Cheney, the vice chairwoman of the Jan. 6 committee, has acknowledged her political peril. A poll released on Thursday by the University of Wyoming’s Wyoming Survey and Analysis Center showed Ms. Cheney trailing Ms. Hageman by nearly 30 points.

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